Granulocyte Marker antibody (CF®740)

Catalog No. ABIN7654656

This anti- antibody is a Mouse Monoclonal antibody detecting in IF, IHC, IHC (fp), WB and RIA. Suitable for Human.

Quick Overview for Granulocyte Marker antibody (CF®740) (ABIN7654656)

Target: Granulocyte Marker

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: CF®740

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Western Blotting (WB), Radioimmunoassay (RIA)

Clone: BM-1

Product Details

Purpose: Granulocyte Marker(BM-1), CF740 conjugate

Characteristics: Recognizes 183 kDa protein with DNA-binding characteristics, which is identified as a myeloid specific antigen. It reacts with myeloid precursor cells and granulocytes in bone marrow. Its antigen appears to be restricted to M2 and M3 acute myelogenous leukemia (AML) subtypes. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. This MAb reacts with early precursor and mature forms of human myeloid cells. It is useful in the identification of myelogenous leukemias, distinguishing granulocytic sarcomas from lymphoid malignancies and also in the study of differentiation and transformation of human myeloid cells. The biological function of this antigen is not clear, although it has been proposed that it may play a role in the differentiation of myeloid cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human peripheral blood mononuclear cells

Isotype: IgG1

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. No special pretreatment is required for staining of formalin/paraffin tissues. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HL60 cells. Bone marrow, lymph node or tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details

Target: Granulocyte Marker

Background:

Synonyms: Myeloid specific protein, p183 DNA-binding cytoplasmic protein

Tissue Expression: Granulocytes|Myeloid cells

Molecular Weight: 183 kDa